Category: Uncategorized

  • COS-1 and COS-7

    COS-1 and COS-7 are immortalized cell lines derived from African green monkey kidney cells (Cercopithecus aethiops). These cell lines were created by introducing Simian Virus 40 (SV40) DNA into primary kidney cells, resulting in the expression of the SV40 large T-antigen. The large T-antigen is known to promote cell immortalization and division, allowing these cell…

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  • DNA Plasmid

    A DNA plasmid is a small, circular, double-stranded DNA molecule that is separate from the chromosomal DNA in a cell. Plasmids are commonly found in bacteria, but can also be present in archaea and eukaryotic organisms. They can replicate independently of the host cell’s chromosomal DNA, and they often carry genes that confer specific traits…

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  • Lipid-based Transfection Reagent

    Lipid-based transfection reagents are a class of reagents used to introduce nucleic acids, such as DNA or RNA, into eukaryotic cells. Transfection is a widely used technique in molecular biology and biotechnology for the study of gene function, regulation, and expression, as well as for the development of gene therapies and recombinant protein production. Lipid-based…

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  • Cell-Growth Regulation

    Cell growth regulation refers to the complex set of processes and molecular mechanisms that control the growth, division, and differentiation of cells within an organism. Proper regulation of cell growth is essential for maintaining tissue homeostasis, preventing diseases such as cancer, and ensuring normal development and function of the body. Cell growth regulation involves numerous…

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  • Simian Cells

    Simian cells refer to cells derived from non-human primates, such as monkeys and apes. These cells have been widely used in scientific research, including virology, immunology, and cell biology, due to their similarity to human cells. Simian cells serve as valuable models to study various aspects of human biology and disease, as they can provide…

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  • Small T-antigen

    The small T-antigen (ST) is a protein encoded by some members of the Polyomavirus family, including Simian Virus 40 (SV40), which is a small, double-stranded DNA virus. Small T-antigen is expressed during the early phase of the viral life cycle, along with the large T-antigen (LT). Although the precise role of small T-antigen in the…

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  • Oncogenic

    Oncogenic refers to the ability of certain agents or factors to cause the formation of tumors or cancer. These agents or factors can be viruses, genes, or environmental exposures, such as radiation or chemicals. Oncogenesis is the process through which normal cells are transformed into cancerous cells, which then proliferate uncontrollably and form tumors. Oncogenic…

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  • Antigens

    Antigens are molecules that can be recognized and bound by the immune system, specifically by antibodies or immune cells, such as T cells. The term “antigen” is derived from “antibody generator,” highlighting their role in stimulating an immune response. Antigens are usually proteins or polysaccharides, but can also be lipids or nucleic acids. Antigens can…

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  • SV40 Contains Genes

    Simian virus 40 (SV40) is a small, double-stranded DNA virus belonging to the Polyomavirus family. It was first discovered in the early 1960s in cultures of rhesus monkey kidney cells and was later found to be a contaminant in some polio vaccines. SV40 is a non-enveloped virus with an icosahedral capsid and has a genome…

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  • Capsid Proteins

    Capsid proteins are the structural proteins that make up the protein coat, or capsid, of a virus. The capsid surrounds and protects the viral genome, which can be composed of DNA or RNA, depending on the virus. They play a critical role in the viral life cycle, including host cell recognition, attachment, entry, and assembly…

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