Cos7 cell line: Transfection protocol, culturing medium, methods

COS (an abbreviation for CV-1 in Origin with SV40 genes) cells are a laboratory cell line derived in the 1960s from monkey kidney tissue. COS cells behave like fibroblasts, and were originally obtained by immortalizing CV-1 cells from the kidney of the African green monkey using a SV40 virus that produces large T antigen but does not replicate correctly. There are several varieties of COS cell lines in common use, including COS-1 and COS-7. COS-7 was developed in the 1980s using transformation with a mutant strain of SV40 coding for the wild-type T-antigen. These cell lines are used both to study SV40, and as a common mammalian production cell for recombinant proteins, including antibodies and signaling molecules.

COS-7 Cell Line Origin:

The COS-7 cell line was derived from the kidney of the African Green Monkey, Cercopithecus aethiops. The cells themselves most resemble fibroblast cells in humans and are thus often called COS-7 monkey fibroblast or COS-7 fibroblast-like cells. COS-7 cells are not primary cells. The CV-1 cell line was initiated in the 1960s from an African Green Monkey Kidney. In the early 1980s, the COS-7 line was developed from the CV-1 line by Yakov Gluzman through transformation with a Simian Vacuolating Virus 40 (SV40), a virus that codes for the wild type virus T-antigen. Using SV40 promoters, a vector can have substantial replication in COS cells using the large T-antigen.

 

cos7 cell culturecos7 cell culture

Copyright picture from altogen.com. Reproduced with permission from Altogen Biosystems.

COS-7 Cell Line Characteristics:

The result of the transformation of CV-1 to create COS-7 was a cell line that expresses the SV40 T-antigen and retains its susceptibility to SV40, which grows lytically within the kidney cells. The replication of the A209 virus at 40C is also permitted, as are SV40 mutants, so long as mutations (deletions) are in the early regions of the viral genome. Thus, COS is an acronym for CV-1 in Origin, carrying SV40.

In culture, COS-7 cells characteristically display adherent growth to glass and plastic surfaces.

Links:

Wikipedia article on Cos-7 cells – COS cells (Wikipedia)

Commercially available Cos-7 cells – ATCC

In Vitro Cos-7 Transfection Reagent from Altogen Biosystems

In Vivo Cos7 siRNA transfection kit – In Vivo Transfection Kits

CRO Pre-clinical Research Services: Cos-7 xenograft animal models

Generation of Stably Expressing Cell Lines in 28 Days

Stable RNAi Cell Line Generation: Stable Gene Knockdown

Transformed African Green Monkey Kidney Fibroblast Cells (COS-7 Line) from the Olympus Confocal Microscopy Image Gallery

COS-7 Transfection Reagent (Kidney Cells, CRL-1651)